Helicobacter pylori infection is a prevalence infection 50% of the human population. The main H. pylori adhesin is the BabA, which was the first identified factor. The aim of this study was to determine the relationship between the ABO blood groups and various gastrointestinal diseases 140 patients, were included in this study. Gastric biopsies were taken for recognition of H.pylori by RUT and PCR. Blood samples were tested for ABO blood group. In the present study 140 H.pylori positive samples examined for the presence or absence of babA gene by PCR. From 140 patients, 35% were positive for babA gene and 65% were negative for this gene. Positivity rate of H. pylori babA infection was 42.4 % in blood group O, 18.8 % in blood group A, 100% in blood group B and 44.8 % in blood group AB.  The frequencies of ABO blood group among endoscopic findings are predominant for Gastritis for group A.  In our study, There was statistically significant difference in babA (+) and babA (–) were compared in endoscopy finding (P<0.001) and there was statistically significant difference in positivity rate of H.pylori infection among ABO blood groups (p< 0.001). The higher incidence of Gastritis and peptic ulcer was in patients with blood group A and AB and there was statistically significant between these symptoms (p= 0.02). Our results showed that the prevalence of babA genotype is associated with gastritis and gastric ulcer and there is a relation with ABO blood group.