ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Lokesha S.1 , Ravi Kumar Y.S.1 , Sonia Gaur2 , Sujan Ganapathy P.S.3 , Arjun H.M.4 and Prashant Gaur5
1Department of Biotechnology, M.S. Ramaiah Institute of Technology, Bangalore – 560 054, India.
2Department of Biotechnology (FET), Manav Rachna International Institute of Research and Studies, Faridabad – 121 004, India.
3Nutrinorm Wellness Pvt.Ltd. #508, 4th Floor, 60ft Road, Sahakaranagar, Bangalore – 560 092, India.
4Nuziveedu Seeds Limited, Survey No-69, Kandlakoya, Medchal Mandal, Hyderabad – 501 401, India.
5Enzibeta Biotech Pvt Ltd.LSI-2, IKP Knowledge Park, Genome Valley, Hyderabad – 72, India.
J Pure Appl Microbiol, 2019, 13 (3): 1847-1854 | Article Number: 5783
https://doi.org/10.22207/JPAM.13.3.62 | © The Author(s). 2019
Received: 16/08/2019 | Accepted: 13/09/2019 | Published: 27/09/2019
Abstract

Betaine is a trimethylglycine, serves as osmoregulator to prevent dehydration and plasmolysis under adverse hyperosmotic environments. Choline oxidase gene from Arthrobacter sp. catalyzes two step oxidation reaction of choline to betaine followed by betaine accumulation in cells which in turn help them to survive and thrive in harsh environmental condition. To identify potential choline oxidase gene source, a gram stain positive, rod shaped, catalase and oxidase positive, motile, aerobic bacterial strains designated as HYJE003 and HYJE005 was isolated based on the colony morphology, biochemical and molecular characterization from the industrially polluted soil samples of Hyderabad, India. Optimum growth of the isolated strains was observed at 32°C on nutrient agar media and was found that both the strains were capable of utilizing variety of sugars as carbon source. The 16S rRNA gene sequence analysis revealed that strain HYJE003 was closely related to Arthrobacter globiformis with pairwise sequence similarities of 99.85%, 99.63%, 98.76% and 98.12% respectively. The strain HYJE005 was closely related to Arthrobacter phenanthrenivorans with pairwise sequence similarities of 99.93%, 99.47%, 99.25% and 98.11% respectively. Choline oxidase gene potential of the isolates was studied by feeding the cultures with choline chloride and converted betaine was assessed by the formation of betaine reineckate. Findings revealed that the isolated strain Arthrobacter globiformis-HYJE003 has four times higher conversion rate of choline chloride to betaine than the strain Arthrobacter phenanthrenivorans HYJE005.

Keywords

Arthrobacter, BLAST, Pleomorphic, Reineckate, Betaine, Snapping division.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.