Journal of Pure and Applied MicrobiologyVol. 1 No. 2

Modeling and Optimization of Synthetic Production Medium for the Production of Xanthan Gum using Response Surface Methodology

V. K. Jain and Mukul Bajpai*

Department of Biochemical Engg. and Food Technology, H.B.T.I., Kanpur - 208 002, India.

Received on 12 August 2007 and accepted on 10 September 2007

 

ABSTRACT

Response surface methodology (RSM) was used to evaluate the effects of variables, namely the concentration of glucose, citric acid, KH2PO4 and NH4Cl, in the synthetic production medium for xanthan production. Central composite design was used in the design of experiments. The experimental results showed that the optimum concentration of the components were (in g/l): glucose, 34.20; citric acid, 2.11; KH2PO4, 4.01 and NH4Cl, 2.13. The maximum xanthan production was 12.5 g/L. This method was efficient; only 24 experiments were necessary to assess these conditions, and model adequacy was very satisfactory as the coefficient of determination was 0.9856.

Keywords : Optimization, central composite design, response surface methodology, xanthan production.