Journal of Pure and Applied MicrobiologyVol. 1 No. 2

Production and Characterisation of Cellulase by Streptobacillus species- aps-8

S.V. Rajagopal1*, K. Siva Kumar, A. Uma Sankar2, M. Rama Rao3 and S. Sunitha Kumari

1Department of Biotechnology, 2Department of Microbiology, 3Department of Biochemistry, College of Science, GITAM University, Visakhapatnam - 530 045, India.

Received on 12 September 2007 and accepted on 17 October 2007

 

ABSTRACT

A cellulolytic bacterial species APS-8 was isolated from the soils of Visakhapatnam region. This strain is characterized as Gram positive Streptobacillus species. The strain grew well in medium containing Carboxymethylcellulose (CMC), producing maximum enzyme at 37C and pH 8.0. The activity of extra cellular enzyme was found to be high at temperature 50C and at pH 5.0. Metal ions like MgSO4, ZnSO4 and FeCl3 enhanced the enzyme activity whereas MnSO4 inhibited the enzyme activity under same assay conditions.

Keywords : Cellulase, Congo red, Carboxymethylcellulose (CMC), Carboxymethylcellulase (CMCase) assay, APS-8.