Journal of Pure and Applied MicrobiologyVol. 1 No. 2

Characterization and Antimicrobial Sensitivity Assay of Gram Negative rods isolated from Neonates with Septicaemia in Zaria

E.E. Ella1*, A.A. Ahmad2, W.N. Ogala3, V.J. Umoh2 and T.B. Balogun3

1Centre for Biotechnology Research and Training, A. B. U. Zaria, Nigeria. 2Department of Microbiology, Ahmadu Bello University Zaria, Nigeria. 3Departmebnt of Paediatrics, Ahmadu Bello University Teaching Hospital Shika, Zaria, Nigeria.

Received on 18 August 2007 and accepted on 27 September 2007



A total of sixty five neonates suspected to be septicaemia were sampled. Nine Gram negative organisms were isolated out of the 40 samples with positive blood culture accounting for 22.5% of the bacterial organisms responsible for neonatal septicaemia within the period of study. Of this number, the most prevalent was Enterobacter cloacae, Enterobacter cloacae accounting for 4 out of a total of 40 patients (10%) and 44.44% of the total Gram negative organisms. Klebsiella pneumoniae was next with an incidence of 7.5% which represents 33.33% of the gram negative organisms. Citrobacter freundii and Escherichia coli had incidence of 2.5% each constituting 11.11% of the total gram negative organism respectively. Incidence was higher in males 55.56% while in females it was 44.44%. The overall sensitivity percentage to ampicillin and gentamicin was 77.78 %, followed by nitrofurantoin , streptomycin and tetracycline which was 66.67%. the percentage sensitivity to colistin was 55.56% while sensitivity to cotrimazole and nalixidic acid was 44.44% respectively. All the Enterobacter isolates were sensitive to ampicillin but one isolate showed multiple resistance to the other antibiotic. the E coli strain also showed evidence of multiple resistance, being resistant to the ampicillin and gentamicin and streptomycin. It was however sensitive to tetracycline and nitrofurantoin.

Keywords : Antimicrogram, Antibiotics, Sensitivity, Septicaemia.