Journal of Pure and Applied MicrobiologyVol. 1 No. 2

Isolation and purification of membrane-bound Cytochrome c and Cytochrome b from Nocardia asteroides

M.Atif Zia1*, M.Shahid Nazir2, Tariq Ismail3, A. Adnan and M.H.Qureshi

1Biochemistry Laboratory (Chemistry Department), 2Microbiology Laboratory (Biotechnology Department), 3Hematology Laboratory (Department of Zoology), Government College University, Lahore, Pakistan.

Received on 10 September 2007 and accepted on 09 October 2007

 

ABSTRACT

In present studies, pathogenic bacterium, Nocardia asteroides was investigated. The growth profile of Nocardia asteroides was studied by optimizing the growth conditions by culturing 20-26 hours with maximum growth. Down-streaming process was accompanied by sonication, differential centrifugation, ultracentrifugation and detergent solubilization techniques. Partially purified membrane-bound proteins were analyzed spectrophotometricaly for membrane-bound protein contents. Reduced membrane spectra and SQR (complex II) activity showed the presence of cytochrome b while pyridine ferrohaemochrome of cytochrome c.

Keywords : Cytochrome b, cytochrome c, respiratory chain, Nocardia asteroides.