Journal of Pure and Applied MicrobiologyVol. 1 No. 2

Visible Spectrophotometric Method for the Determination of a Lipid lowering drug in Pharmaceutical Dosage forms

S. Vijaya Saradhi1*, G. Devala Rao2, P. Srinivasu2 and B.S.S.Venkatesh2

1Department of Biotechnology, Koneru Lakshmaiah College of Engineering, Vaddeswaram – 522 502, India. 2K.V.S.R. Siddhartha College of Pharmaceutical Sciences, Vijayawada - 520 010, India. 3P.B. Siddhartha College of Arts and Science, Vijayawada - 520 010, India.

Received on 20 September 2007 and accepted on 02 October 2007

 

ABSTRACT

A simple, sensitive and reproducible spectrophotmetric method is developed for the determination of Atorvastatin calcium in bulk and dosage forms. This method is based on the formation of colour species on binding of Atorvastatin calcium with potassium ferro cyanide and ferric chloride to produce pale green coloured solution (l max at 425 nm). Results of analysis were validated statistically and by recovery studies. This method is successfully employed for the determination of Atorvastatin calcium in various pharmaceutical preparations.

Keywords : Atorvastatin calcium, Visible Spectrophotometric determination, Beer’s Law.