Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Draft Genome Sequence of the Endophytic Bacterium Pantoea ananatis MR5 Isolated from Cenchrus biflorus- A Drought Tolerant Plant

Disheeta L. Akbari, B.A. Golkiya and Roshani A. Bhadania

Department of Biotechnology, Junagadh Agriculture University, Junagadh, India.

Received on 08 April 2015 and accepted on 11 June 2015

 

ABSTRACT

An endophytic plant growth promoting bacteriumPantoea ananatis MR5 was isolated from roots of Cenchrus biflorus, a drought tolerant plant. Genome sequencing of Pantoea ananatis MR5 was carried out in Ion Torrent (PGM), Next generation Sequencer. The data obtained revealed 242 contings with genome size of 4.1 Mb and G+C content of up to 55%.The size of cell was measured with average of 1.56┬Ám by using Scanning electron microscope.This bacterium had contain genes responsible for drought tolerance in plant, including phosphate solubilisation, IAA, siderophore production and ACC deaminase activities for plant.

Keywords : Drought tolerance endophyte, Plant growth promoting bacterium, Scanning electron microscope, Whole genome sequencing.