Journal of Pure and Applied MicrobiologyVol. 9 No. 3

A Systematic Approach for Optimization of Up-scaling the Expression and Kinetic Characterization of Recombinant Plasmodium falciparum Lactate Dehydrogenase (PfLDH) in E.coli

Saurabh Shrivastava1, Anil K. Singh1, Kshitij Chandel2, Ritu Gill3, Prativa K. Behara4 and N. Gopalan2*

1Bio-process Scale up Facility, Defence Research and Development Establishment, Ministry of Defence (Govt. of India), Gwalior - 474 002, India. 2Vector Management Division, Defence Research and Development Establishment, Ministry of Defence (Govt. of India), Gwalior - 474 002, India. 3Centre for Biotechnology, MD University, Rohtak-124001, India. 4ISPAT General Hospital, Rourkela - 769 005, India.

Received on 03 May 2015 and accepted on 03 July 2015

 

ABSTRACT

Plasmodial lactate dehydrogenase enzyme is known to be a potential immunodiagnostic marker as well as a novel target for chemotherapy and hence its economical bulk production is required. Although Escherichia coli offers a mean for rapid, high yield, and economical production of recombinant proteins, high-level production of functional recombinant Plasmodium falciparum lactate dehydrogenase enzyme in E. coli is quite challenging. To explore a new approach for high level soluble expression of PfLDH in E. coli, the recombinant clone carrying the transgene was subjected to optimizations of expression conditions at shake flask level. The comparative analysis of different culture parameters revealed that modified-TB medium at a specific pH has the highest capacity to produce the high level of expression of PfLDH. There was an increase of 23% pure protein from the culture grown under optimised conditions in comparison to normal medium. The purified rPfLDH was found to be biologically active with specific activity of 485.3 ┬Ámol/min/mg. We believe that this strategy could be of special interest due to its capacity to improve the expression level of PfLDH and the procedure described in this study may provide a reliable and simple method for production of large quantities of soluble and biologically active PfLDH.

Keywords : Malaria, PfLDH, optimized expression, affinity purification, enzyme kinetics.