Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Molecular Typing of Salmonella Typhimurium and S. Enteritidis Serovars from Diverse Origin by ERIC-PCR

Anjay1, Ashok Kumar1*, R.K. Agarwal2, T.P. Ramees1, Z.B. Dubal1, P. Kaushik3, M. Suman Kumar1, N.C. Dudhe1, A.A.P. Milton1, Abhishek2, B. Kumar4 and Shagufta Bi1

1Division of Veterinary Public Health, Indian Veterinary Research Institute Izatnagar, Bareilly - 243122, India 2Division of Veterinary Bacteriology and Mycology, Indian Veterinary Research Institute Izatnagar, Bareilly - 243122, India 3Department of Veterinary Public Health, Bihar Veterinary College Patna, Bihar - 800014, India 4Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, Bareilly (U.P.) – 243122, India

Received on 15 April 2015 and accepted on 03 June 2015

 

ABSTRACT

Food borne infections due to Salmonella spp. are of high concern around the globe. The present study was undertaken with the aim of genotyping of Salmonella Typhimurium and S. Enteritidis from different sources by enterobacterial repetitive intergenic consensus- polymerase chain reaction (ERIC-PCR). A total of 69 strains comprising S. Typhimurium (49) and S. Enteritidis (20) obtained from the National Salmonella Centre, Indian Veterinary Research Institute, Izatnagar and Department of Veterinary Public Health, Bihar Veterinary College, Patna were subjected to molecular typing by ERIC-PCR. The isolates were revived and confirmed by PCR targeting invA, typh and ent genes along with biochemical characterization. Based on the results of ERIC-PCR, S. Typhimurium isolates were categorized into 6 clusters (C1 to C6) with a discriminatory power (D) of 0.7764, while S. Enteritidis into 4 clusters (B1-B4) with a discriminatory power of 0.7368. Dendrogram analysis of S. Typhimurium and S. Enteritidis revealed 60% and 71% similarity, respectively between isolates. The isolates under study from different sources like animals, vegetables, humans, sewage, water and wild animal sources showed similar band patterns. The study revealed that Salmonella serovars share common source contamination and confirmed the zoonotic nature of this important pathogen with global significance.

Keywords : Salmonella Typhimurium, S. Enteritidis, ERIC-PCR, dendrogram, discriminatory power.