Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Screening and Plant Growth Promoting Activity of Drought Tolerant Endophytic Bacteria Isolated from Wild Poaceae

Disheeta L. Akbari*, B.A. Golakiya and Roshani A. Bhadania

Department of Biotechnology, Junagadh Agriculture University, Junagadh - 362 001, Gujarat, India.

Received on 25 May 2015 and accepted on 03 July 2015

 

ABSTRACT

A total 26 unique bacterial endophytic strains were isolated from roots, stems and leaves of Dactyloctenium sindicum, Cenchrus biflorus and Chloris barbata plants and eleven isolates were screened as a drought tolerant endophytic bacteria. The CEB 9 and CEB 76 drought tolerant isolates were positive for phosphate solubilisation, seven endophytic isolates were positive to siderophore and eight isolates were positive to ACC deaminase activity. The CEB 76 isolate produced 47.96 µgml-1 Indole acetic acid at 30% PEG 6000 concentration. While the CEB 9 isolate produced 154.68 µgml-1 of exopolysaccharide at 30% PEG6000. The CEB 12, CEB 15 and CEB 75 isolates were successfully inhibited growth of Alternaria triticina and Helminthosporium sativum. The CEB 76 isolate only inhibited growth of Helminthosporium sativum. This study evaluates the drought tolerant capacity of endophytic bacteria, plant growth promoting activity and biocontrol mechanism of drought tolerant bacteria.

Keywords : Drought tolerant endophytic bacteria, PGPR, Antagonistic potential.