Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Isolation, Characterization and Screening of Phosphate Solubilizing Bacteria

Fozia Shafiq Wani1, Tahir Ali1 and Zahoor Ahmad Baba2

1Division of Soil Science, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar- 191 121, India. 2Organic farming research center, Wadura Campus, Sher-e-Kashmir University of Agricultural Sciences and Technology Kashmir, Sopore-193 201, India.

Received on 10 January 2015 and accepted on 11 March 2015

 

ABSTRACT

Thirty phosphate solubilizing bacteria (PSB) were isolated from rhizosphere of maize crop from different maize growing areas of Kashmir valley by serial dilution technique using Pikovskaya’s agar medium and were compared with one another for P-solubilization, production of iaa and ga (growth promoting substances), siderophore production and ammonia production. The PSB (K4) proved to be the most efficient strain and was identified as Pseudomonas sp. On screening the isolate recorded maximum phosphorus solubilization (60.00 µg ml-1), produced highest amount of IAA (36.07 µ mol ml-1) and GA (38.20 µ mol ml-1) which was significantly superior over all other isolates and thus can be exploited as biofertilizer. The isolate also showed ammonia and high siderophore production.

Keywords : characterization, Isolation, Kashmir, Phosphate solubilizing bacteria, Screening.