Journal of Pure and Applied MicrobiologyVol. 9 No. 3

A Combined Morpho-Molecular Approach towards Identification of Chaetomium Species of India

V. Chandra Sekhar, T. Prameeladevi and Deeba Kamil

Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi-110012, India.

Received on 28 April 2015 and accepted on 06 July 2015

 

ABSTRACT

The Chaetomium, recently identified as a bio control fungal genus includes many species. The classification of the Chaetomium species based on morphology alone is very difficult as this genus has multiple species-specific characters in several species. Therefore, an attempt has been made to confirm the identity of Chaetomium species by integrating morphological and molecular (using Internal Transcribed Spacer region; ITS) characters of different species of Chaetomium. Sixty one isolates were morphologically characterized using perithecium, terminal hairs, lateral hairs, rhizoids and ascospores. The identity of these isolates was confirmed using ITS region based molecular characters. The taxonomy of nine valid species of the Chaetomium were described here.

Keywords : Chaetomium, Morphological characterization, ITS region, Identification.