Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Therapeutic Efficacy of Lytic Bacteriophage PSAE-1 against Salmonella Abortusequi in Guinea Pig Model

Abhishek1, R.K. Agarwal1, B. Kumar2, A.K. Mishra3, Anjay4, A. Priyadarshini1, S. Kumar1, C. Prakash1, J.K. Chaudhary5 and M. Rawat6

1Division of Veterinary Bacteriology and Mycology, Indian Veterinary Research Institute Izatnagar, Bareilly (U.P.) – 243122, India. 2Division of Biological Products, Indian Veterinary Research Institute Izatnagar, Bareilly (U.P.) – 243122, India. 3Goat Health Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122. 4Division of Veterinary Public Health, Indian Veterinary Research Institute Izatnagar, Bareilly (U.P.) – 243122, India. 5Division of Livestock economics, statistics and Information Technology Indian Veterinary Research Institute, Izatnagar, Bareilly (U.P.) – 243122, India. 6 Division of Biological Standardization, Indian Veterinary Research Institute Izatnagar, Bareilly (U.P.) – 243122, India.

Received on 07 April 2015 and accepted on 16 June 2015

 

ABSTRACT

Global emergence of multi drug resistance (MDR) against the bacterial pathogens has motivated scientists to exploit alternative strategies to combat the problem. Under these demanding circumstances, therapeutic uses of bacteriophages may turn out to be a valuable approach. The present study was planned to evaluate therapeutic efficacy of Salmonella Abortusequi phage (PSAE-1) in experimentally infected female guinea pig. Phage PSAE-1 was characterized in terms of lytic host range and multiplicity of infection (MOI). Broad host range phage having MOI of 1:104 was used for therapeutic trial. Safety of phage was tested in experimental animal for any toxicity and it was found safe through various routes. Therapeutic efficacy of PSAE-1 was assesed in female guinea pig at different time intervals after infection. Average total spleen counts of Salmonella Abortusequi strain-156 (SAE-156) were enumerated after the phage treatments. There was significant increase in therapeutic response of phage treated groups in comparison to untreated control group. There was no significant effect on animal health following phage treatment. Present study suggests that phage may be used in future as a therapeutic preparation to reduce the colonization/infection of Salmonella Abortusequi and could be a novel approach in treatment of infections associated with Salmonella spp.

Keywords : Salmonella Abortusequi, Bacteriophage, Guinea pig, Therapeutic.