Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Optimization of Pigment Production by a Novel Bacillus sp. BBMRH Isolated from Cow Dung

S.K. Mondal, D.P. Samantaray* and B.B. Mishra

Department of Microbiology, Orissa University of Agriculture and Technology, Bhubaneswar- 751 003, India.

Received on 28 April 2015 and accepted on 01 July 2015



Cow dung has substantial potential for microbial diversity. On account of that an attempt was made to study the total aerobic heterotrophic bacteria of cow dung procured from local market of Bhubaneswar, Odisha. Out of 15 bacterial isolates, CD-5 showed deep red pigmentation in nutrient broth culture medium. The biochemical characterization of red colour pigment producing bacterial isolate resembled to the genus Bacillus. Moreover, significant pigment production was observed at pH 7.0 ±0.1 and temperature 34ºC. The extracted pigment showed maximum similarity with Rhodamine-6G as revealed from absorption spectrum. The potent bacterial isolate was identified by 16S rRNA gene sequencing and found to be Bacillus sp. with accession number KF175230.

Keywords : Pigmentation, Bacillus, 16S rRNA, Rhodamine-6G, Absorption.