Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Degradation of Monocrotophos by Aspergillus oryzae SJA1 isolated from Oryza sativa Field Soil

Anudurga Gajendiran and Jayanthi Abraham*

Microbial Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore - 632014, India.

Received on 02 February 2015 and accepted on 09 March 2015

 

ABSTRACT

Monocrotophos (MCP) is an organophosphours pesticide and controls the variety of pest in the agricultural fields. Fungal strain capable of degrading monocrotophos was isolated from Oryza sativa field soil. The SJA1 strain was identified based on 18S rRNA sequence analysis and it was designated as Aspergillus oryzae SJA1. The degradation process was studied by high performance liquid chromatography (HPLC). Aspergillus oryzae SJA1 degraded 500 mg/L monocrotophos in the aqueous medium as well as soil. The two metabolites such as diethylcyanamide and benzene 1,3-bis(1,1-dimethylethyl) were obtained during the degradation of monocrotophos. Besides, plant growth promoting traits such as phosphate solubilizing activity was also studied. The present study explores the potential of Aspergillus oryzae SJA1 in degrading monocrotophos which can be further used for remediating soil contaminated with MCP.

Keywords : Aspergillus oryzae SJA1, Monocrotophos, Mycoremediation, Phosphate solubilization.