Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Antagonistic Activity of Indigenous Strains of Pseudomonas fluorescens Against Fungal Plant Pathogens

Narinder Singh1*, Moumita Ghosh1, Daljeet Singh1, Shiveta Raina1 and Ahmed Ibrahim A.I. Heflish2

1Department of Plant Pathology, Punjab Agricultural University, Ludhiana - 141004, India. 2Department of Agricultural Botany, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria, Egypt.

Received on 11 April 2015 and accepted on 09 June 2015

 

ABSTRACT

Twelve indigenous strains of Pseudomonas fluorescens from different soils of Punjab were isolated to see the antagonistic activity against different fungal pathogens. Significant amount of IAA was produced by isolate PSF 1 (22.12 µgmL-1) followed by PSF 4 (13.66 µgmL-1), PSF 12 (12.41 µgmL-1) and PSF 5 (12.09 µgmL-1). All the isolates showed HCN positive reaction whereas PSF 1, PSF 3, PSF 8 and PSF 12 showed highest HCN production. Maximum siderophore production was by PSF 12 (18.7 mm) followed by PSF 5 (16.5 mm) and PSF 7 (16.0 mm). PSF 5 and PSF 12 showed positive chitinase production. Except PSF 1 all the culture were positive for starch hydrolysis with a maximum zone by PSF 12 (22.0 mm) followed by PSF 10 (21.0 mm). For protease production all the culture showed positive results with a maximum zone of solubilization by PSF 12 (30.5 mm) followed by PSF 5 (29.5 mm) and PSF 1 (29.3 mm). In general PSF 12 followed by PSF 5 and PSF 7 showed maximum inhibition percentage against the different fungal pathogens tested in vitro confirming Pseudomonas fluorescens a good antagonist.

Keywords : Pseudomonas fluorescens, IAA, HCN, siderophore, antagonist.