Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Purification of PCR Products Using Bandstab PCR Method: Application to Short 16s-23s Intergenic Spacer Region of Lactobacillus Spp

Tanedjeu Sonfack Kemgang1,2*, Huguette Gaelle Ngassa Mbenda3, Suman Kapila1 and Rajeev Kapila1

1National Dairy Research Institute, Animal Biochemistry Division, 132001 Karnal, Haryana, India. 2University of Ngaoundere, National School of AgroIndustrial Sciences, Department of Food Science and Nutrition, PO Box 455, Ngaoundere, Adamaoua, Cameroon. 3National Institute of Malaria Research, Evolutionary Genomics and Bioinformatics, 110077 New Delhi, Delhi, India.

Received on 10 May 2015 and accepted on 03 July 2015

 

ABSTRACT

In view to show a simple DNA purification step from agarose gel, we applied the DNA bandstab technique to the 16S-23S Intergenic Spacer Region (ISR) (a polymorphic region resulting in multiple bands after gel-electrophoresis) of selected Lactobacillus spp. The short ISR was successfully extracted by bandstab after numerous unsuccessful attempts of DNA cutting-elution techniques. The purification of band was further confirmed by the distinctive peaks observed in the DNA chromatograms generated after the sequencing procedure.

Keywords : Bandstab-PCR; Lactobacillus; 16S-23S intergenic spacer region; Sequencing.