Journal of Pure and Applied MicrobiologyVol. 9 No. 3

High-Efficient Regeneration and Transplanting for Industrialized Seedling Production of Biomedical Dendrobium officinale

Jie-Ming Qin, Dang-Quan Zhang*, Fang-Yuan Wu, Han-Jie He, Rong Chen and Xiao-Lei Zhang

Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees (Ministry of Education) / Hunan Provincial Key Laboratory of Forestry Biotechnology / Cooperative Innovation Center of Cultivation and Utilization for Non-Wood Forest Trees of Hunan Province, Central South University of Forestry and Technology, Changsha 410004, China.

Received on 28 April 2015 and accepted on 03 July 2015

 

ABSTRACT

Dendrobium officinale was widely used as a rising valuable forest Chinese medicinal herb in recent years in China. By the systematical researches on seed aseptic germination, protocorm induction and proliferation, rooting and hardening-off culture, seedling transplanting and tending, the highly efficient regeneration system and domestication transplanting technology for D. officinale were established. The results showed that 1/2 MS without any phytohormone is the optimal medium for seed aseptic germination of D. officinale, the medium suitable for protocorm induction and proliferation is MS + 1.0 mg/L 6-BA (6-Benzylaminopurine) +1.0 mg/L NAA (1-Naphthaleneacetic acid) +1.0 mg/L KT (kinetin) according to the orthogonal tests, the optimal medium for rooting and hardening-off culture of D. officinale is 1/2 MS+ 0. 2mg /L IBA (3-indolebutyric acid) + 0.5% activated carbon + 40% potato extract, and the corresponding seedling transplanting medium is the 1:1:5 combination of vermiculite, perlite and humus soil. The high-efficient regeneration and transplanting technique obtained will contribute to the industrialized seedling production of Biomedical D. officinale.

Keywords : Dendrobium officinale; Regeneration system; Seedling transplanting; Medicinal herb, Biomedicine.