Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Identification of Dermatomycoses Pathogens by Multilocus Sequence Typing Method

Elena V. Kukhar1, Ainur M. Sharipova1 and Alexander B. Shevtsov2

1S.Seifullin Kazakh Agro Technical University, 62, Prospect Pobedy, Astana, Kazakhstan, 010000. 2National Center for Biotechnology MES RK, 13/1, Valikhanova Str., Astana, Kazakhstan, Astana.

Received on 04 March 2015 and accepted on 03 May 2015

 

ABSTRACT

Identification of dermatomycetes with the use of traditional methods is often problematic. This is due to microscopic or macroscopic atypical morphological features that occur when cultured on nutrient media. The purpose of this study was to evaluate the possibility of using multilocus sequence typing using primers ITS region (ITS4/ITS5) and SSU region (NS1/NS4), RPB1, RPB2 to identify dermatomycoses pathogens. Regions of DNA dermatomycetes were amplified by PCR and it was sequenced. Nucleotide sequences were analyzed and combined in a common sequence which was identified in the GeneBank by BLAST algorithm. According to fungi identification except classical dermatomycoses pathogens, they are mold and yeast.

Keywords : Multilocus sequence typing, Dermatomycetes, Identification, Primers, ITS region.