Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Effect of the Formulation of Seaweed (Porphyra umbilical R.) in Biopreparations based on Trichoderma harzianum Rifai

Amaro Leal J.L.1, Romero-Arenas O.2*, Rivera A.3, Huerta L.M.4 and Reyes E.5

1Manejo Sostenible de Agroecosistemas; Benemerita Universidad Autónoma de Puebla (BUAP), Mexico. 2Centro de Agroecología; Instituto de Ciencias,BUAP. 3Centro de Investigaciones en Ciencias Microbiologicas ICUAP-BUAP. 4Departamento de Desarrrollo Sustentable ICUAP-BUAP. 5Laboratorio de Investigacion Aplicada del Centro Universitario de Vinculacion, BUAP.

Received on 06 April 2015 and accepted on 19 August 2015

 

ABSTRACT

The use of biological control to reduce the incidence of diseases originated in soil is an alternative technique to using synthetic fungicides. Among the most important agents are fungi of the genus Trichoderma, which are able to control a large number of phytopathogens in plants of agricultural interest. However, the productions of biofungicides have restrictions on the viability and optimal concentration of conidia, as well the potential that may have native strains is unknown. In the current investigation, a hydratable biological fungicide was developed based on the fungus Trichoderma harzianum Rifai to assess the viability and germination capacity of conidia with 90 days of storage at 18 °C. The 4 biopreparations were obtained from organic and inorganic compounds, plus a native strain of Puebla-Mexico, from an eroded soil of the municipality of Tetela de Ocampo. The formulation based on seaweed, corn starch, zeolite and conidia of the TH-CA1 strain, obtained a concentration of 83x104 / mL with 98% viability, reduced viability of conidia was obtained based on zeolite biopreparation, which was observed through a microphotograph (3000 X) a lot of amorphous and collapsed conidia. These results demonstrate the feasibility of using seaweed (Porphyra umbilical R.) as a preservative in the formulation of hydratable biopreparations Trichoderma harzianum based powder.

Keywords : Trichoderma harzianum, native strain, concentration of conidia and microphotography.