Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Identification of Bacteria from the Rhizosphere of Jatropha curcas with Characteristics of Biotechnological Interest

Arnoldo Wong-Villarreal1*, Fernando Kante-Lopez1, Ana L. Reyes-Reyes2, Ricardo Sanchez-Cruz1, Antonio De Leon-Martinez1, Gustavo Yanez-Ocampo3, Saul Espinosa-Zaragoza4, Orlando Lopez-Baez5 and Nancy S. Hernandez-Bueno6

1Division Agroalimentaria; Universidad Tecnologica de la Selva, Ocosingo, Chiapas, Mexico. 2Programa Bioenergia; Campo Experimental Rosario Izapa, INIFAP 3Ingenieria en Tecnologia Ambiental; Universidad Politecnica de Chiapas, Tuxtla Gutierrez, Chiapas, Mexico. 4Facultad de Ciencias Agricolas, Universidad Autonoma de Chiapas, Huehuetan, Chiapas, Mexico. 5AUDES Cacao-Chocolate, Centro Universidad Empresa de la Universidad Autonoma de Chiapas. Tuxtla Gutierrez, Chiapas, Mexico. 6Centro de investigacion en Biotecnologia, Universidad Autonoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.

Received on 16 June 2015 and accepted on 16 August 2015

 

ABSTRACT

In the present study Pseudomonas sp. S18 (PMa-76), Pseudomonas aeruginosa N7B1 (PMa-83), Pseudomonas sp. (PMa-88), Pseudomonas nitroreducens TX1 (PMa-89), Pseudomonas strains sp. bf1 (PHx -97), Pseudomonas aeruginosa F23 (PHx -98), Pseudomonas aeruginosa F23 (PHx -101) strains were isolated from the rhizosphere of physic nut. The PMa-83 PMa-88 PMa-89, PHx -97, PHx -98, PHx -101 strains grew up in the presence of phenol 0.05% and benzene 1.0%, as a sole carbon source, as well as in the presence of zinc, copper and cobalt in concentrations of 0.1 and 5 mg/ml. The Pseudomonas sp. S18 (PMa-76) strain was the one that had the highest capacity to solubilize phosphate (9.6 mm). Moreover indole production in 6 strains were detected and while biosurfactants production was detected in 5 strains. Furthermore, the presence of nifH genes in PMa-83, PHx -97, PHx-98 and PHx P-101 strains were also detected, indicating the ability to fix nitrogen. PMa 83 is the strain in which the growth in phenol, benzene and metals were detected, thus indole production, the presence of biosurfactants and nifH genes which are characteristics of biotechnological interest.

Keywords : Phosphate solubilization, Jatropha curcas, indoles, biosurfactants.