Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Antilisterial Effects of Ziziphora clinopodioides Essential Oil and Nisin in Milk

Yasser Shahbazi

Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.

Received on 09 April 2015 and accepted on 10 June 2015



The aim of the present study was to evaluate the antibacterial effect of Ziziphora clinopodioides essential oil (0.1 and 0.2%) separately and in combination with nisin (250 and 500 IU/ml) on survival of Listeria monocytogenes inoculated in pasteurized milk sample during storage under refrigerated temperature for 9 days. Chemical composition of the essential oil was analyzed by gas chromatography coupled with mass spectrometer detector (GC-MS). Carvacrol (64.22%), followed by thymol (19.22%) and p-cymene (4.86%) were the most abundant components of the essential oil. Based on our findings, there was no significant difference (p>0.05) between samples treated with the essential oil at 0.1 and 0.2% and also nisin at 250 and 500 IU/ml. Our samples treated with the essential oil in combination with nisin showed the populations of pathogen significantly (p<0.05) lower than the untreated samples. Moreover, samples treated with the combination of the essential oil at 0.2% and nisin at 500 IU/ml, showed populations of L. monocytogenes significantly (p<0.05) lower than those of samples treated with other groups. According to the results of this work, Z. clinopodioides essential oil and nisin could be applied in food industries as alternative food preservatives and to control the growth of L. monocytogenes.

Keywords : Milk, nisin, Listeria monocytogenes, Ziziphora clinopodioides essential oil.