Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Polymorphisms at Exon 4 of the Prolactin Gene in Najdi and Naeimi Sheep of Saudi Arabia

Ahmed Hossam Mahmoud

Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh11451, Saudi Arabia.

Received on 27 April 2015 and accepted on 12 June 2015

 

ABSTRACT

Prolactin (PRL) hormone plays an important role in many biological processes in animals and humans, such as mammogenesis, lactogenesis and galactopoiesis. PRL is a single-chain protein of 199 amino acids (AA) in human, sheep, pigs and cows. The objective of the present study was to determine the polymorphism in exon 4 of PRL gene in 41 Najdi and 36 Naeimi sheep breeds of Saudi Arabia. A fragment of 156 bp of exon 4 of the Prolactin gene was amplified and sequenced. Our mutations were detected at positions (05, 109 and 127) of exon 4. In addition, the three mutations were used to construct different haplotypes, four haplotypes were recovered, H1 (CGG), H2 (CGT), H3 (AGG) and H4 (CCG). The H1 (CGG) was the most common haplotype and found in Najdi and Naeimi individuals with frequencies of 0.927% and 1.00% for Najdi and Naeimi sheep breeds, respectively. The haplotypes H2, H3 and H4 were unique for Najdi sheep with frequency of 0.025% for each. Naeimi sheep breed was monomorphic at exon 4 of the Prolactin gene. The present study provides basic information to understand the genetic Characterization of local sheep breeds in Saudi Arabia.

Keywords : Prolactin gene, Najdi and Naeimi sheep, Single Nucleotide Polymorphism.