Journal of Pure and Applied MicrobiologyVol. 1 No. 2

Reduction of Chromium by Microbial Method: Transmission Electron Microscopic Study using Electron Energy Loss Spectroscopy in-situ Environmental Cells

Wani P. P., A.C. Sonawane and S.R. Thorat*

School of Environmental and Earth Sciences North Maharashtra University, Jalgaon, India.

Received on 28 August 2007 and accepted on 05 October 2007



In the present study, we present the indirect Cr6+ reduction by Shewanella oneidensis using sulphur as energy source under aerobic and anaerobic conditions and the subsequent Cr3+ precipitation by Shewanella oneidensis under anaerobic conditions. Intermediate products such as sulfite and thiosulfite promote Cr6+ reduction. Both processes have been operated sequentially under continuous flow conditions to decontaminate 5mg/l-1 Cr6+ solutions. The associated mechanism of Cr6+ reduction are technological and biological importance because the convert a toxic, mobile element into less toxic, immobile form. The results obtained through this investigation indicates possibility of capability to determine chemistry and valance state of reduction product associated with hydrated bacteria in more of less either than natural state i.e. (No. TEM specimen preparation) in an environmental cells.

Keywords : Spectroscopy, electron energy, Hexavalent chromium, in-situ environmental cells.