Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Molecular Identification of Drought Tolerant Endophytic Bacteria from Grasses of Kutch by Sequencing of the 16s rRNA Coding Gene

Disheeta L. Akbari*, B. A. Golakiya and Roshani A. Bhadania

Depatment of Biotechnology, Junadadh Agricultural University, Junagadh, Gujarat, India.

Received on 01 April 2015 and accepted on 09 July 2015

 

ABSTRACT

Endophytic bacteria from seven species of grasses native to the banni region of Kutch (Dactylactenium sindicum, Cenchrus biflorus, Sporobolus fertilis, Alelurops lagopoides, Eleusine indica, Chlorius barbata and Cenchrus ciliaris), were isolated and identified, through partial sequencing of the 16s rRNA encoding gene. The sequence of 17 isolates deposited in NCBI and compared to sequences- 29.41% belonged to the genus Bacillus; 17.64% to Staphylococcus; 11.76% both to Methylobacterium and Pseudomonas, Where as 5.88% were Brevibacillus, Enterobacter, Pantoea, Ochrobacterium and Micrococcus. Results obtained through sequence analysis revealed high genetic diversity across the isolates. This is the first report concerning the isolation and identification of drought tolerant endophytic bacteria in these grass species of banni region from Kutch.

Keywords : Grasses, Drought tolerant endophytic bacteria, 16s rRNA Sequencing, NCBI, BLASTn.