Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Prevalence of Nonfermenters in Pyogenic Infections with Antimicrobial Profile

Sanjay Kumar Sarkar and Debadatta Dhar (Chanda)

Department of Microbiology, Silchar Medical College & Hospital, Silchar, Assam - 788014, India.

Received on 08 July 2015 and accepted on 19 August 2015



The non-fermentative Gram negative bacilli (NFGNB) are a group of aerobic, non-spore forming bacilli that either do not use carbohydrates as a source of energy or degrade them through metabolic pathways other than fermentation. The present study was planned to identify the non-fermenters isolated from pyogenic infections and to know their antimicrobial sensitivity pattern. A total numbers of 300 pus samples were collected from the cases of pyogenic infection, in Silchar Medical College & Hospital during the period from June 2013 to May 2014. The nonfermenters were identified by standard conventional laboratory procedure and oxidation-fermentation etc. Antimicrobial sensitivity pattern was determined by Kirby-Bauer disc diffusion method. Out of 300 pus samples, 56 (18.7%) were isolated as nonfermenters. The most common species identified was Pseudomonas aeruginosa 40 (71.4%) followed by Acinetobacter baumannii 10 (17.9%), Pseudomonas fluorescens 5 (8.9%) and Pseudomonas putida 1(1.8%). The in vitro antimicrobial sensitivity test showed 100% sensitivity by Polymyxin- B against nonfermenters isolated in the present study followed by Imipenem (82.1%), whereas Levofloxacin showed least sensitivity (33.9%). Nonfermenters are fairly common organisms associated with pyogenic infections in this region as observed in the present study. The most common isolates were Pseudomonas aeruginosa followed by Acinetobacter baumannii. The most effective antibiotics were Polymyxin-B, followed by Imipenem.

Keywords : Nonfermenters, Gram negative bacilli, Pyogenic infection, Imipenem.