Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Isolation, Identification, Characterisation and In silico Analysis of Pseudomonas stutzeri VITNR-1 from Amirthi Forest Soil, India

D. Nancy VeenaKumari, T.B. Sridharan and Mohana Priya Arumugam

School of Bio Sciences and Technology, VIT University, Vellore - 632 014, India.

Received on 06 April 2015 and accepted on 19 August 2015

 

ABSTRACT

The aim of this study was to isolate, identify, Characterize and in silico analysis of a novel bacterial strain from the Amirthi forest that is located in Vellore district of Tamil Nadu. The soil sample were serially diluted and plated on Nutrient agar. The inoculated plates were incubated at 37 °C for 24-48 hrs. A total of 22 isolates were obtained, out of which 12 were bacteria. From these ten different colonies of various morphology and pigmentation was selected for the study. Then the isolates were characterised by morphological, biochemical and molecular techniques. One of the isolate showed wrinkled dry morphology on Nutrient agar. It was further subcultured on selective medium. The microbiological analysis, 16 S rRNA followed by in silico analysis showed that the isolate found to be Pseudomonas stutzeri. The isolate is submitted in Gen bank under the accession no FR848954 Pseudomonas stutzeri VITNR-1. The accession no of other isolates are KM047486 Bacillus subtilis VITNJ1, KM047487 Bacillus sp VITNJ2, KM047488 Bacillus licheniformis VITNJ3, KM047489 Bacillus sp VITNJ4, KM047490 Bacillus pseudomycoides VITNJ5, KM047491 Arthrobacter nicotianae VITNJ6, KM047492 Rummeliibacillus stabekisii VITNJ7, KM04749 Enterobacter sp. VITNJ8, KM047494 Bacillus safensis VITNJ9. Further Pseudomonas stutzeri VIT NR-1 isolate was checked for antibiotic sensitivity test and in vitro antimicrobial activity for pathogens like E.coli, Bacillus subtilis ,Klebsiella, Proteus, P.aeruginosa, and Staphylococcus.

Keywords : Antibiotic resistance, in silico analysis, Nutrient agar, Pseudomonas stutzeri, Rhizosphere , 16 S rRNA.