Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Molecular Characterization of Antagonistic Bacteria, Pseudomonas otitidis from Insect gut, Short horned Grasshopper

C.Chellaram1* and M. Mark Praveen2

1Department of Biomedical Engineering, Vel Tech Multitech, Chennai-600062. Tamilnadu India. 2St. Peter's University, Avadi. Chennai-600062. Tamilnadu. India.

Received on 09 April 2015 and accepted on 06 June 2015

 

ABSTRACT

The aim of the present work is to investigate bioactive metabolites from gut bacteria of short horned grasshoppers. In this present study the bacteria are isolated from the gut of short horned grasshoppers and screened for antagonistic activity. To test for antagonistic effect the gut isolated bacteria were tested against four human pathogenic bacteria Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli, Vibrio parahameolyticus and one yeast like fungus Candida albicans. It was observed that gut associated Pseudomonas sp had antagonistic activity against yeast like fungus Candida albicans and Escherichia coli. The most potent strain was subjected for 16srRNA gene sequencing in order to identify the antagonistic bacteria. Phylogenetic tree has been constructed and our strain matches with Pseudomonas otitidis (99.1%). Pseudomonas otitidis has found to possess antimicrobial activity and the active compounds isolated can be utilized as antibiotics.

Keywords : Antagonistic bacteria, short horned grasshoppers, 16srRNA, Pseudomonas otitidis.