Journal of Pure and Applied MicrobiologyVol. 9 No. 3

Facile Approaches for Microbial DNA Extraction: Comparative Study with Colonies “Picked” from three Different Bacteriological Mediums

J.R. Parvathi and S. Singh*

School of Biotechnology and Bioinformatics (SBB-DYP), D.Y. Patil University, Maharashtra, India.

Received on 10 April 2015 and accepted on 12 June 2015

 

ABSTRACT

DNA isolation preludes any nucleic acid amplification method but also tend to increase the time duration depending on the procedure adapted. The current work is aimed at swotting simplistic methods involving mechanical shearing, boiling and alkaline lysis for bacterial DNA extraction. Comparative analysis of the DNA yield from bacterium “picked” from three different mediums were also tried to select the optimal practice for productive results. Apart from being an expeditious step, Boiling lysis (BL)was also proved to be efficacious for further analysis using PCR-RFLP.

Keywords : DNA extraction, String test, boiling lysis, Selective and differential medium, PCR-RFLP.