Aqueous two-phase Extraction of Intracellular Lipase with Sn-1,3 Positional Selectivity from A. niger GZUF36

ISSN: 0973-7510

E-ISSN: 2581-690X

Aqueous two-phase Extraction of Intracellular Lipase with Sn-1,3 Positional Selectivity from A. niger GZUF36

Chao-Shuang Xia^1,2, Yu-Di Wu^1,2, La-Ping He^1,2,3,
Fu-Hao Zhang^1,3, Cui-Qin Li⁴, Yi-Ming Zhang¹ and Li Chen¹

¹College of Liquor and Food Engneering, Guizhou University, Guiyang 550025, China

²Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy,
Guiyang – 550025, China.

³Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy,
Guiyang – 550025, China.

⁴School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China.

J Pure Appl Microbiol. 2015;9(Spl. Edn. 1):177-181

Received: 16/02/2015 | Accepted: 20/04/2015 | Published: 31/05/2015

Abstract

The extraction of intracellular lipase with Sn-1,3 positional selectivity from a new strain of A. niger GZUF36 has been exploited by aqueous two-phase system (ATPS). Firstly, crude intracellular lipase solution was prepared by cell disruption. Sonication is the best method for disruption of A. niger cell. Then, extraction of the intracellular lipase from the crude enzyme solution was focused on by ATPS. The influence of key parameters such as molecular weight of polyethylene glycol (PEG), PEG 2000 concentration and (NH4)2SO4 concentration on the partitioning behavior of lipase was evaluated. The suitable aqueous two-phase system consisted of 20% PEG 2000 and 12% (NH4)2SO4 resulted in one-sided partitioning of the intracellular lipase with selectivity of 2.39, recovery of lipase 74.93% and purification factor of 3.42-folds. ATPS extraction may be used as a first step to purify the enzyme from crude enzyme solution.

Keywords

Sn-1,3 position selectivity; intracellular lipase; A. niger GZUsF36; aqueous two phase system; extraction

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