In this study to be tried that check out the effect of Satureja khuzistanica essential oil (SKEO) against mexXY-oprM and mexEF-oprN efflux pumps genes expression in MDR and clinical isolates of Pseudomonas aeruginosa. For notice of the genes expression amount before and after of confronting with SKEO, genotyping and phenotyping methods were used. In phenotyping method was applied MIC technique with specific antibiotic markers (Norfloxacin and Imipenem for MexEF-OprN and Gentamicin for MexXY-OprM). In genotyping method was used of RT-PCR technique. The amount of SKEO,s MIC compared to antibiotics were much less. The MIC of antibiotic markers and intensity of bands on gel electrophoresis after confronting decreased. Reduce in MIC of antibiotic markers and as well as in the bands intensity show that SKEO decreased expression pumps and both methods confirm each other. Therefore, SKEO could be an important factor in the reduction of antibiotic resistance in the P. aeruginosa isolates. According to these results, we expect be applied SKEO as a supplement therapy in future infections.
Genotypic and phenotypic methods, SKEO Effect, Pseudomonas aeuroginosa
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