ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Jenifer Selvarani A.1, Nishanthini P.1, Raji P.1, Sree Samanvitha K.2 , Ponnaiah Paulraj3, Iyappan P.3, Chandramohan M.3, and Antony V. Samrot3 
1Department of Biotechnology, School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, Sholinganallur, Rajiv Gandhi Salai, Chennai, Tamil Nadu – 600 119, India.
2Department of Biotechnology, Shanmugha Arts, Science, Technology & Research Academy, Thanjavur, India.
3Department of Biomedical Sciences, Faculty of Medicine and Biomedical Sciences, MAHSA University, Jalan SP2, Bandar Saujana Putra, 42610, Jenjarom, Selangor, Malaysia.
J Pure Appl Microbiol, 2019, 13 (3): 1863-1876 | Article Number: 5863
https://doi.org/10.22207/JPAM.13.3.64 | © The Author(s). 2019
Received: 28/08/2019 | Accepted: 21/09/2019 | Published: 30/09/2019
Abstract

Besides being an essential source of nutrients, the bioactive components of some fruits also help in enhancing the physiological functions by expressing its therapeutic action, acting as radical scavenger, improving digestion and healing. Here, seven edible fruits juices of Punica granatum, Citrus reticulata, Anana scomosus, Ficus carica, Vitis vinifera, Vitis amurensis and Carica papaya were utilized against biofilm forming Gram negative bacteria, Pseudomonas aeruginosa to evaluate its effect on Quorum sensing. On proving its antibacterial activity and anti-swarming motility in our earlier report, this work is extended to determine the biofilm inhibitory action of these fruit juices due to the impact on AHL (Acyl Homoserine lactone), the signaling molecule responsible for developing cell-cell communication and also on AHL mediated metabolites production. The fruit juices were evaluated for their Antioxidant activity on subjecting to TLC bioautography, DPPH and FRAP assay. AHL, Pyocyanin and Rhamnolipid were extracted from fruit juices treated Pseudomonas aeruginosa and the influence of fruit juice was identified by FT-IR and LC-MS analysis. However, AHL production was not stopped by fruit juice molecules but showed least production level in Punica granatum treated P.aeruginosa. Whereas the production of pyocyanin pigment was disturbed in Punica granatum, Citrus reticulata, Vitis amurensis and Vitis vinifera treated culture. The production of a biosurfactant called Rhamnolipid (Rha 10) was a failure in Citrus reticulata, Vitis amurensis, Vitis vinifera and Ficus carica treated P.aeruginosa. Thus the organism was restrained by the fruit juice molecules from expressing its virulence factors in spite of having no impact on AHL synthesis.

Keywords

Quorum sensing, biofilm, AHL, virulence factors, Pseudomonas aeruginosa.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.