Three different organic solvents which include hexane, chloroform and methanol were used to extract the bioactive compounds from the root of Asparagus racemosus to screen the antimicrobial activity against selected plant, aqua and human clinical pathogens by agar diffusion method. The methonolic extract of A. racemosus was more active against 90% of the organisms tested. It was followed by chloroform extract (75%) and hexane extract (20%) in inhibiting the growth of the organisms tested. The crude extracts of A. racemosus had broad spectrum of activity because they inhibit the growth of both gram positive, gram negative bacteria and fungal pathogens as well.