Enterococci are part of the normal flora of humans and animals. Recently, Enterococci have caused great concern due to developing of resistance to many antimicrobial agents. Their ubiquity in animal and human digestive tracts ,  medical importance,  frequent multiple  antibiotic  resistance and their seemingly unlimited capacity for horizontal gene transfer via numerous mobile genetic elements make this bacterial group ideal for investigating the ecology of antibiotic resistance.  The aim of this study was to investigate and identify the prevalence of VRE (Vancomycin Resistant Enterococcus) within isolated Enterococci taken from a number of Tehran Livestock husbandry units. Putative Enterococci (n=242) were isolated on Membrane Filter Enterococcus Selective Agar Medium , supplemented with 2 , 4 and 8 µgr/ml vancomycin from cow samples.  A total isolates passed the standard biochemistry tests for the genus and species as well as specific genus and species primers.  The antibiotic susceptibility was determined by the disc diffusion method for 6 antibiotics. MIC of vancomycin was also done using broth micro-dilution assay by CLSI recommendations. Results showed that   138,  90  and 14 of the isolates  were E. faecium , E. feacalis and E. gallinarum,  respectively.  41, 25, 18, 10, 21 and 22 of the isolates were resistant to vancomycin , tetracycline, gentamicin , chroramphenicol, ciprofloxacin and erythromycin, respectively.  An MIC test on 65% of the isolates was >256 µgr/ml. Diversity of VRE isolates was restricted to 3 species. E. faecium had high resistance to a broad range of antibiotics. The results of this study suggest that more attention should be paid to the livestock samples   as a reservoir of resistance elements. Surveillance of VRE reservoirs in animal husbandry to clarify the mechanism of transfer are urgently required.