The present study was under taken to detect biofilm production in nosocomial isolates of Pseudomonas aeruginosa and EDTA- antibiotic combination induced dispersal and killing of Pseudomonas cells in biofilm. Different clinical samples were collected from site of infection judged on the basis of clinical findings. P. aeruginosa was isolated and identified by standard conventional methods. Biofilm production was detected by Congo Red Agar (CRA) method and further antibiogram was analyzed in presence of antibiotics as well as in combination with EDTA by disc diffusion method and Agar well diffusion technique respectively.

               Amongst the 100 samples, 80 Pseudomonas isolates were obtained and screened for biofilm detection. Maximum isolates (85%) were found able to grow within slime by CRA method. Most of the isolates exhibited resistance towards Penicillin suggesting that its further use may lead enhancement of drug resistance disaster. Increased in diameter of zone of inhibition was recorded in presence of combination revealing its better utility for treatment purpose. The combination of EDTA-Gentamicin and EDTA-Tobramycin significantly inhibited Pseudomonas growth. The present study demonstrated that EDTA is a potent P. aeruginosa biofilm disrupter. EDTA treatment of Pseudomonas biofilm results in 1000 fold greater killing than treatment with antibiotics alone.  A combination between proper amounts of EDTA with antibiotics will improve the control of Pseudomonas aeruginosa biofilm.