The emergence of vancomycin- resistant Staphylococcus aureus (VRSA) in various parts of the world has been of great concern in clinical settings. This study was carried out to isolate pathogenic S. aureus from wounds pus sample, and VRSA was identified by evaluation of resistance patterns using conventional antibiotics and vancomycin by PCR. A total 54 S. aureus were isolated from samples. All S. aureus were subjected to MIC testing (against vancomycin), Brain Heart Infusion (BHI) vancomycin screen agar test, disc diffusion testing.VRSA were confirmed by PCR amplification of the vanA and vanB genes.  From this study, it was observed that isolated S. aureus strains are pathogenic, Using the disk diffusion test, most isolates (89.3%) were resistant to penicillin while the lowest resistance (7.9%) was to cephalothin. Three of the 54 isolates had a vancomycin MIC of ³ 128 by MIC testing. All three VRSA isolates were also highly resistant to other examined antibiotics. The present study reveals for the first time emergence of VRSA from this part of Iran and indicates the antibiotic resistance in the study area.