ISSN: 0973-7510
E-ISSN: 2581-690X
The incidence of AFB1 (B1) was determined in 72 wheat derivatives samples (Biscuits, bread, cornflakes, flour, macaroni and wheat bran. Positive sample for biscuits, bread and flour (16.7%), cornflakes (8.33%), macaroni (33.33%) and wheat bran (50%). Sixteen samples were contaminated with B1 ranging from 0.21 to 2.17 µg/kg. PCR was used for the detection of B1 contamination based on aflatoxin biosynthetic genes aflR, aflS, aflD, aflM and aflP. The interpretation of the results revealed that PCR is a rapid and sensitive method.
AFB1, Wheat derivatives, aflR, aflS, aflD, aflM and aflP
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