J Pure Appl Microbiol | Research Article | Volume 12, Issue 4 | Article Number: 5347

Prabin Kumar1,2, Pragya Misra1, Abhinav Saurabh1,2, Narayan Rishi2 and Dipendra Kumar Mitra1

1Department of TII, AIIMS, New Delhi – 110 029, India. 2Amity Institute of Virology and Immunology, Amity University, Noida – 201 303, India.

Corresponding Author E-mail: raiprabin79@gmail.com
Received: 13/11/2018| Accepted: 19/12/2018 |Published: 30/12/2018
DOI: http://dx.doi.org/10.22207/JPAM.12.4.10

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Abstract

Containment/dissemination of Leishmaniasis depends on the dominance of Th1/ Th2 immunity. IFN-g and IL-17A are well known for protection from leishmaniasis. Synergistic effects of these two cytokines are also known in various autoimmune diseases. However, the therapeutic, as well as adjunct therapeutic use of rIL-17A and rIFN-g in combination with sub-optimal dose of amphotericin-B (AmpB) is still not validated in visceral leishmaniasis. In the present study, we have evaluated the adjunct therapy in a mouse model of VL. After twenty-one days of post infection, in the therapeutic group, mice were intra-peritoneally injected with two doses of recombinant cytokines at one week interval. In adjunct therapeutic groups of mice, immune components were primed for three days with recombinant cytokine(s) followed by injection of sub-optimal dose of AmpB. Body weight, parasitic load in visceral organs and fold change in cytokines’ gene expression was evaluated. We observed significant gain in body weight, inhibition of parasitic load in visceral organs {(liver; 71.7% – 95%), (spleen; 70%-88.7%) (Bone marrow; 46.6 -87.1%)}; significant up regulation in fold change of pro-inflammatory cytokine(s) gene expression (TNF-g,iNOS, IL-2 and IL-12) as well as marginal increase of anti-inflammatory cytokine(s) gene expression (IL-4, IL-10&TGF-g} in adjunct therapeutic groups of mice. Our results suggest that though the therapeutic use of recombinant cytokine(s) is not the best option; however, use of recombinant cytokine(s) along with suboptimal dose of amphoterocin-B to reduce drug toxicity could have a way for better treatment options.

Keywords: Balb/c mice, IL-17, therapeutic, adjunct therapy, Visceral Leishmaniasis.