The hydrophobic/hydrophilic and electron donor/electron acceptor characters for Escherichia coli AL52 were determined using microbial adhesion to solvents method (MATS). The glass with adhered cells was observed using scanning electron microscope (SEM) and the SEM images were processed with Mathlabâ program to quantify the adhesion to glass.  Strong adhesion was found at pH 2. For pH 3 the adhesion was relatively high but for other pH it was low or null. The results also show that the electron donor character plays a crucial role in microbial adhesion of Escherichia coli to glass. Moreover the role of hydrophilic-hydrophilic interactions is not always sufficient to explain adhesion between two hydrophilic surfaces. Also our finding shows that the types of interactions governing microbial adhesion to glass depend on pH of suspending medium.