J Pure Appl Microbiol | Research Article | Volume 12, Issue 4 | Article Number: 5318

Ramu Satheesh Murugan1,2, Gujuluva Hari Dinesh1,2, Tondi Rajan Angelin Swetha2, Thulasinathan Boobalan2, Muthuramalingam Jothibasu3, Panneer Selvam Manimaran4, Gopal Selvakumar5 and Alagarsamy Arun2*

1Department of Energy Science, Alagappa University, Karaikudi – 630003, India.
2Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi – 630 003, Tamil Nadu, India.
3Department of Botany, DDE, Alagappa University, Karaikudi – 630 003, India.
4Department of Botany, Alagappa University, Karaikudi – 630 003, Tamil Nadu, India.
5Department of Microbiology, DDE, Alagappa University, Karaikudi – 630 003, Tamil Nadu, India.

Corresponding Author E-mail: arunalacha@gmail.com
Received: 19/09/2018| Accepted: 20/11/2018 |Published: 31/12/2018
DOI: http://dx.doi.org/10.22207/JPAM.12.4.09



The present study aims to enhance the efficiency of anaerobic sludge microorganisms to produce hydrogen (H2) through various pre-treatment methods. The various pre-treatment methods such as base, acid, chloroform, heat shock, freezing and thawing have enabled to isolate acidogenic bacteria with higher bio H2 producing activity in an anoxic environment. From these various treatments, bacteria were isolated and screened for bio H2 capabilities. Among the one bacterial strain, AH4 strain showed maximum cumulative H2 production and Hydrogen Yield (HY) using 100% diary anaerobic sludge. AH4 strain was identified as Acinetobacter junii using 16S rRNA gene sequence and used for further experimental analysis. Biohydrogen productions of Acinetobacter junii were measured at different experimental setup such as various pH levels (5.0, 5.5, 6.0, 6.5, 7.0, 7.5 and 8.0) and different substrate concentration (10 – 100%) of dairy anaerobic sludge substrate. At pH 7.5 and 60% substrate concentration, the strain AH4 Acinetobacter junii displayed the maximum cumulative H2 production of 945.7 ml/L and H2 yield 1.35 mol H2/mol glucose. Based on our results, we concluded that Acinetobacter junii can be used as a promising bio agent for hydrogen production on a large scale using dairy anaerobic sludge as substrate.

Keywords:  Bio hydrogen, Dairy industry anaerobic sludge, Pretreatment, Optimization, Batch experiment.