Urinary tract infections (UTIs) are commonly prevalent in obstetrics and gynaecology settings and Indian data from this specific patient population are lacking. This retrospective study collected microbiological urine reports from Obstetrics and Gynaecology Department over a period of two years. A total of 5903 urine samples were screened and 383 bacterial isolates were identified using standard microbiological techniques and antimicrobial susceptibility was performed. Of the 383 isolates, 77.02% (295/383) were from OPD patients and 22.97% (88/383) from IPD patients. Escherichia coli and Klebsiella spp. were the two most common uropathogens, their prevalence being 225/383 (58.7%) and 40/383 (10.4%), respectively. E. coli showed 100% susceptibility to fosfomycin in both OPD (193/193) and IPD (32/32) patients. Klebsiella spp. showed 0% (36/36) and 25% (1/4) susceptibility to nitrofurantoin among OPD and IPD isolates, respectively. Our study emphasizes the importance of microbiological report in UTI caused by Klebsiella spp. prior to nitrofurantoin empirical therapy which is largely ineffective in our geographical settings.