Blood is a body fluid. When it contacts oxygen, it can clot, forming a dark solid substance. In accidents involving wounds, blood is released and can stain clothing when absorbed by fabrics. Removing blood stains from fabrics is challenging with various commercial detergents. This study aimed to determine the efficacy of commercially available detergent powder supplemented with amylase and protease enzymes in removing bloodstains from textiles. Stutzerimonas xanthomarina and Pseudomonas pseudoalcaligenes were isolated from soil and examined for their enzymatic activity. The results demonstrated that these enzymes can effectively remove bloodstains from cotton fabric, with maximum activity observed at 50% saturation with ammonium sulfate and specific activity of protease at 93.45 ± 2.17 U/mg and amylase at 36.28 ± 1.36 U/mg. This study suggests that the addition of enzymes to chemical detergents can enhance their surface impact and improve their effectiveness. These findings indicate a potential for the development of more efficient detergents.