Salt pan water collected from Kovalm, Kanyakumari district, Tamil Nadu, used for bacterial isolation using Zobal marine agar. Red pigmented colonies were selected for further studies and labelled as KSB. Microscopic, macroscopic and molecular studies were performed for the identification of isolate and it is confirmed as Delftia. Gen Bank accession number obtained for the same and deposited as Delftia MW172212. The optimization studies concluded that optimum temperature was 40 °C, optimum pH was 8 and optimum lactose concentration was 20%. Acetone precipitation concentrates active compounds, increasing efficacy against Gram-negative bacteria, according to the Delftia sp. acetone-precipitated fraction, which had a maximal inhibitory activity against E. coli MTCC-1671 that was marginally greater than the pure isolate (17 mm). Neutral red assay showed significant inhibition against MCF-7 hence it is considered as anticancer compound.