Bloodstream infections caused by bacteria can cause potentially fatal sepsis, which needs immediate antibiotic therapy to prevent morbidity and death of patient. Blood culture remains the gold standard procedure that provides the vital information for the diagnosis and guiding appropriate antimicrobial treatment. The study sought to assess the antibiotic susceptibility and bacteriological profile of blood culture isolates in intensive care unit (ICU) settings. Understanding the incidence of diverse bacteria in ICU blood cultures, as well as their antibiotic susceptibility, is crucial for developing effective treatment plans that work. During the study period, 3,594 blood cultures underwent analysis, revealing 388 cases positive for growth. To identify isolates, VITEK 2 GN ID cards were utilized, capable of discerning both fermentative and non-fermentative bacteria. Further, VITEK 2 GP ID was employed for selected Gram-positive cocci. Antibiotic susceptibility testing was conducted using VITEK 2 AST 407 Critical Care cards for fermentative and non-fermentative Gram-negative bacilli, with subsequent testing on VITEK 2 AST 628 cards for Gram-positive cocci. Out of which, 230 (59.2%) were Enterobacteriaceae isolates, 87 (22.4%) were non-fermenters, and 71 (18.2%) were Gram-positive cocci. The majority of the blood culture isolates were multidrug-resistant (MDR), extended-spectrum beta-lactamase (ESBL) producers, carbapenemase producers, and methicillin-resistant Staphylococcus aureus (MRSA). In our study, we observed carbapenem-resistant Klebsiella spp., Escherichia coli (E. coli), Acinetobacter baumannii, Pseudomonas aeruginosa, and Burkholderia cepacia, which is alarming. The results show the diverse range of microorganisms responsible for bloodstream infections in severely ill ICU patients. Understanding the antibiotic susceptibility characteristics of these isolates is crucial for developing effective therapeutic regimens.