ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Arifatun Nasicha1, Ni Made Mertaniasih2,3,4 , Eko Budi Koendhori2,3, Pepy Dwi Endraswari3,4, Ariani Permatasari5 and Atika6
1Resident of Clinical Microbiology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia.
2Department of Clinical Microbiology, Dr. Soetomo Hospital, Surabaya, Indonesia.
3Department of Clinical Microbiology and Parasitology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
4Department of Clinical Microbiology, Universitas Airlangga Hospital, Surabaya, Indonesia.
5Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
6Department of Public Health and Preventive Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Article Number: 10094 | © The Author(s). 2025
J Pure Appl Microbiol. 2025;19(2):1134-1144. https://doi.org/10.22207/JPAM.19.2.16
Received: 23 November 2024 | Accepted: 21 March 2025 | Published online: 21 May 2025
Abstract

Tuberculosis (TB) is an infectious illness induced by Mycobacterium tuberculosis (MTB), a principal cause of mortality globally. The incidence of TB in Indonesia is increasing every year. WHO recommends Xpert MTB/RIF Ultra, which is sensitive and specific, as an initial method of TB diagnosis that detects IS1081/IS6110 of the Mycobacterium tuberculosis complex (MTBC). The study intends to analyze the accuracy of Xpert Ultra with Mycobacterial Growth Indicator Tube (MGIT) 960 culture system of pulmonary TB in the Clinical Microbiology Laboratory of Dr. Soetomo Academic Hospital, Indonesia. This was an analytical, observational, and cross-sectional study that included 39 of 382 sputum samples that met the inclusion and exclusion criteria from suspected pulmonary TB patients. The result showed that 56.4% (22/39) were adults, and 41% were elderly (>60 years), 61.5% (24/39) were male, and 43% of them were smokers. Demographic factors, epidemiology, patients’ clinical conditions, and chest x-ray patterns all have no significant value on suspected pulmonary TB cases (p > 0.05). Xpert Ultra sensitivity was 83.3%, specificity 82.5% and accuracy 84.6%. There are no statistical differences between the two diagnostic methods (p = 0.687, McNemar’s). In conclusion, Xpert Ultra can diagnose pulmonary TB as well as MGIT 960 system culture. Although demographic factor, epidemiology, clinical symptoms, and also chest x-ray pattern cannot confirm the diagnosis of pulmonary TB, it is necessary to carry out confirmation tests under the guidelines.

Keywords

Tuberculosis, Xpert MTB/RIF Ultra, MGIT 960 Culture System, Chest X-ray

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© The Author(s) 2025. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.