ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Helen J. Lawalata1 , Ni Wayan Suriani2, Soenandar M. Tengker3,Jenny Kumajas3, Susanly4, Indri Kereh4 and Christy Tumanduk5
1Department of Biology, Faculty of Mathematics, Natural Science, and Earthly, Manado State University, North Sulawesi Province, Indonesia.
2Departement of Natural Science, Faculty of Mathematics, Natural Science, and Earthly, Manado State University, North Sulawesi Province, Indonesia.
3Department of Chemistry, Faculty of Mathematics, Natural Science, and Earthly, Manado State University, North Sulawesi Province, Indonesia.
4Graduate Student Majoring in Biology, Faculty of Mathematics, Natural Science, and Earthly, Manado State University, North Sulawesi Province, Indonesia.
5Student of Majoring in Biology, Faculty of Mathematics, Natural Science, and Earthly, Manado State University, North Sulawesi Province, Indonesia.
Article Number: 9967 | © The Author(s). 2025
J Pure Appl Microbiol. 2025;19(1):658-668. https://doi.org/10.22207/JPAM.19.1.55
Received: 08 October 2024 | Accepted: 04 January 2025 | Published online: 04 March 2025
Issue online: March 2025
Abstract

The formation of extracellular polysaccharide polymers (EPS) is catalyzed by the enzyme Fructosyltransferase (ftf), derived from lactic acid bacteria (LAB). These enzymes produce various homopolymers, including fructans, levans, inulin, and fructooligosaccharides (FOS), which are beneficial as prebiotics, emulsifiers, stabilizers, and gelling or water-binding agents in food products. In the health sector, they also exhibit potential antitumor, antiulcer, and immunomodulatory properties and help lower cholesterol levels. This study aims to identify lactic acid bacteria as probiotic candidates encoding the fructosyltransferase gene (ftf) from Pakoba fruit (Syzygium sp.). The methods used in this study included reculturing six isolates of probiotic candidate LAB-EPS, DNA isolation, amplification of the 16S rRNA gene using universal primers (27F and 1492R), and amplification of the ftf gene using specific primers (5FTF and 6FTF). The amplification of the 16S rRNA gene produced amplicons of approximately 1400 bp, while the amplification of the ftf gene yielded amplicons of approximately 800 bp. Two of the six probiotic LAB-EPS isolates were found to harbor the ftf gene: the PM6.4 and PM5.3 isolates. Identification based on the 16S rRNA gene sequence revealed that the PM6.4 and PM5.3 isolates belong to the species Lactiplantibacillus plantarum. This study concludes that the two LAB-EPS probiotic isolates from Pakoba fruit (Syzygium sp.) contain the FTF gene, identified as Lactiplantibacillus plantarum.

Keywords

Lactic Acid Bacteria, Pakoba, Fructosyltransferase, Lactiplantibacillus plantarum

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© The Author(s) 2025. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.