ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Mingzhu Zheng1,2, Jia Lao3, Zhenni Xie1,2, Wei He3, Can Zhong2,
Changzheng Xu3, Xiaofang Zhang3, Shuihan Zhang2 and Jian Jin1,2
1Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China.
2Institute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha, Hunan 410013, China.
3Resgreen Group International Inc., Changsha 410329, China.
Article Number: 10141 | © The Author(s). 2025
J Pure Appl Microbiol. 2025;19(1):647-657. https://doi.org/10.22207/JPAM.19.1.54
Received: 10 December 2024 | Accepted: 05 February 2025 | Published online: 03 March 2025
Issue online: March 2025
Abstract

This study investigated the storage temperature effects on Polygonati Rhizoma probiotics (PRP) activity by establishing storage conditions at different temperatures and regularly detecting the viable bacteria counts and value of pH. To further analyze the metabolic changes, the metabonomics analysis was carried out by using UHPLC-QE-MS/MS. Multivariate analysis used principal component analysis and orthogonal partial least squares discriminant analysis. When the storage temperatures were 25 °C and 37 °C, 229 and 307 different metabolites were identified respectively. The levels of most compounds such as glutamine, asparagine, and citrate decreased. This change affected both the flavor and nutritional benefits of PRP. In addition, the count of viable bacteria at 20 °C and 4 °C is very high, and there is no significant difference. Therefore, considering the product quality and energy saving, we recommend 4 °C as the storage temperature. This research provides important information on the storage stability and shelf-life setting of PRP products.

Keywords

Polygonati Rhizoma, Traditional Chinese Medicine, Probiotic, Stability, Shelf Life, Metabolomics, Storage Method

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© The Author(s) 2025. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.