Aflatoxins (AFs) are the most potent and ubiquitously found mycotoxins, capable of causing contamination in agricultural products. Aflatoxin B₁ (AFB₁) is the most toxic and primarily produced Aflatoxin and will be a real threat to the safety of food and feeds. The current study searched for the potential of Lactic acid bacteria (LAB) isolated from animal excreta for AFB₁ mitigation. Three LAB out of 56 isolates were found to exhibit more than 50% sorbent action with AFB₁ in phosphate-buffered saline (PBS) and were identified as Lactococcuslactis strain CF_6 (OP183481) (65.38%), Lacticaseibacillus casei strain CW_3 (OP183482)(52.63%) and Lactobacillus acidophilus strain CE_4 (OP183483)(63.13%). More than 60% of the total AFB₁ removal was observed in 2 hr of incubation, and maximum sorbent action was found at a pH 6-7 range at 37^oC for 24 hours. In the Scanning Electron Microscope (SEM) analysis, heat-killed cells showed a significant increase in cell surface binding area, which improved the surface binding for all isolates except L. casei strain CW_3; however, it proves that LAB surface binding is strain-specific rather than heat treatment. Moreover, the rise in AFB₁ concentration improved the rate of the sorbent action but did not observe any substantial changes in total AFB₁ detoxification. So, it is concluded that the animal excreta may be a versatile source of probiotic LAB for AFB₁ detoxification by surface binding.