ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Nikky Nyari Srivastava1, Harjeet Singh Maan2 , T.N. Dhole1, Jasmeet Singh1, Swati Sharma1, Shyam Narain Pandey3 and Ankita Agarwal2
1Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
2State Virology Laboratory, Department of Microbiology, Gandhi Medical College, Bhopal, Madhya Pradesh, India.
3Department of Botany, Lucknow University, Uttar Pradesh, India.
Article Number: 8234 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(2):1141-1153. https://doi.org/10.22207/JPAM.17.2.44
Received: 11 November 2022 | Accepted: 12 April 2023 | Published online: 30 May 2023
Issue online: June 2023
Abstract

Dengue is one of the most critical diseases, caused by Dengue virus (DENV) serotypes (DENV-1 to DENV-4). Study aims to detect DENV in natural A. aegypti from endemic regions of Uttar Pradesh. From 2010- 2013, mosquitoes collected from Uttar Pradesh tested for Dengue virus serotypes by capsid-premembrane gene-based PCR analyzed for genotypes sequencing of the C-prM junction of DENV genome. A total of 4731, 53.54% (n=2671) A. aegypti and 46 % (n=2060) A. albopictus mosquitoes were collected. Of 226 mosquito pools, 10 pools of A. aegypti and 14 pools of A. albopictus were positive for DENV by PCR. All 24 isolates identified as DENV-I; Genotype (G)-III (n=8), G-V (n=1); DENV-2; G-IV (n=4); DENV-3; G-III (n=11). The overall minimum infection rate was much higher in A. albopictus mosquitoes and presence of MIR in male mosquitoes is an indicating natural vertical transmission and important observation in geographical area indicating natural vertical transmission.

Keywords

Aedes Mosquitoes, Entomological Surveillance, Dengue Virus, Vertical Transmission, Polymerase Chain Reaction

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